Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination. Bi C, et al. Plant Mol Biol, 2017 May. PMID 28391398, Free PMC Article; Disruption of AtWNK8 enhances tolerance of Arabidopsis to salt and osmotic stresses via modulating proline content and activities of catalase and peroxidase.

2019-02-01

In Arabidopsis, the basic leucine zipper transcription factor ABI5 activates several late embryogenesis–abundant genes, including AtEm1 and AtEm6 . However, the expression of many other seed maturation genes is independent of ABI5 . We investigated the possibility that ABI5 homologs also participate in the regulation of gene expression during seed maturation. We identified 13 ABI5 -related ABI5 | Abscisic acid insensitive 5 (peptide antibody) Product information Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence,UniProt: Q9SJN0, TAIR: AT2G36270 Host Rabbit Clonality Polyclonal Purity Affinity purified serum in PBS, pH 7.4 Format Lyophilized Quantity 100 µg ABI5.

In addition to effector genes involved in seed maturation and storage, several signaling proteins and transcription factors not previously known to be involved in the ABA signaling pathway were identified as targets of ABI4 and/or ABI5. negative regulation of seed germination Source: TAIR "The gibberellic acid signaling repressor RGL2 inhibits Arabidopsis seed germination by stimulating abscisic acid synthesis and ABI5 activity." Piskurewicz U. , Jikumaru Y. , Kinoshita N. , Nambara E. , Kamiya Y. , Lopez-Molina L. Plant Cell 20:2729-2745(2008) [ PubMed ] [ Europe PMC ] [ Abstract ] ABI5-BINDING PROTEIN2 (AFP2) negatively regulates the abscisic acid signal by accelerating ABI5 degradation during seed germination in Arabidopsis ( Arabidopsis thaliana ). The abscisic acid signal is reported to delay flowering by up-regulating Flowering Locus C expression, but the role of AFP2 in regulating flowering time is unknown. Here, we found that flowering time was markedly delayed ABI5 overexpression rescued ppc2 seedling growth arrest under low CO 2 conditions. It has been reported that ABI3, ABI4, and ABI5 (the downstream targets of the ABA signaling pathway) are required for ABA modulation of seed germination and post-germination development (Finkelstein and Lynch, 2000; Lopez-Molina et al., 2001). The abi5-5 mutation was characterized at the molecular level and was shown to result from a two base pair deletion in the coding sequence of the ABI 5 gene. The wild type and mutant recombinant proteins were produced in E. coli and were assayed for DNA-binding activity on their target promoters by electrophoretic mobility shift assay (EMSA).

ABI5 | Abscisic acid insensitive 5 (peptide antibody) Product information Immunogen KLH-conjugated peptide derived from Arabidopsis thaliana ABI5 sequence,UniProt: Q9SJN0, TAIR: AT2G36270 Host Rabbit Clonality Polyclonal Purity Affinity purified serum in PBS, pH 7.4 Format Lyophilized Quantity 100 µg ABI5.

2020-09-07 · ABI5 is a central regulator of seed germination and post-germinative growth in Arabidopsis (Lopez-Molina et al., 2001). To test whether ABT affects early developmental processes through ABI5, we performed western blot analysis to assess ABI5 protein levels in imbibed abt-1D, abt-2, and WT seeds treated with ABA

The wild type and mutant recombinant proteins were produced in E. coli and were assayed for DNA-binding activity on their target promoters by electrophoretic mobility shift assay (EMSA). 2019-02-01 Transgenic lines with similar amounts of ABI5 (En‐2 Super:ABI5‐MYC vs bes1‐D Super:ABI5‐MYC) were selected as a pair, and two independent pairs were selected and used for the following studies (Fig. S2a,b). Transgenic lines Col‐0 35S:ABI5‐MYC and bes1 Super:ABI5… Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of growth.

It is expressed in seeds and up-regulated in response to ABA. Loss of function mutants show decreased rate of germination in the presence of ABA. ABA dependent regulation is mediated by both ABI3 and ABI5. ABI5 promotes MFT expression, primarily in the radicle-hypocotyl transition zone and ABI3 suppresses it in the seed.

ABA not only activates ABI5 transcription, but also promotes ABI5 accumulation (Finkelstein and Lynch 2000; Lopez‐Molina et al. 2001). Reduced transcription of FLC results, at least partially, from the abnormal splicing and reduced transcript abundance of ABSCISIC ACID‐INSENSITIVE 5 (ABI5), which encodes an activator of FLC in ABA‐regulated flowering signaling. Additionally, the expression of AtU2AF65b is promoted by ABA. ABI5 is a central regulator of seed germination and post-germinative growth in Arabidopsis (Lopez-Molina et al., 2001). To test whether ABT affects early developmental processes through ABI5, we performed western blot analysis to assess ABI5 protein levels in imbibed abt-1D, abt-2, and WT seeds treated with ABA for 48 h. Abitare Blog is a lifestyle blog focused on Interior Design.

It is expressed in seeds and up-regulated in response to ABA. Loss of function mutants show decreased rate of germination in the presence of ABA. ABA dependent regulation is mediated by both ABI3 and ABI5. 2008-12-16 Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes. The dgat1 mutant (CS3861) was purchased from TAIR.
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Background ABI5 (abscisic acid insensitive 5) is involved in ABA-regulated gene expression during seed development and subsequent vegetative stage and acts as the major mediator of ABA repression of growth. Binds to the embryo specification element and the ABA-responsive element (ABRE) of the Dc3 gene promoter and to the ABRE of the Em1 and Em6 genes promoters. JMJ17-WRKY40 and HY5-ABI5 modules regulate the expression of ABA-responsive genes in Arabidopsis THE NEW PHYTOLOGIST AT1G63490 AT1G80840 AT2G36270 AT5G11260 Under these conditions, ABI4 and ABI5 each induced a small set of genes (95 and 59, respectively), with only 16 shared targets.

ABA INSENSITIVE 5, F2H17.12, F2H17_12, GIA1, GROWTH-INSENSITIVITY TO ABA 1, AT2G36270. protein abscisic acid-insensitive 5.
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ABI5 is a key component in ABA-triggered pathways during germination, seedling establishment, and vegetative growth (Lopez-Molina et al., 2001); in addition, it has been reported to play certain roles in nitrogen assimilation and signaling (Signora et al., 2001).

Responsible for reducing cadmium uptake, mediated by interaction with MYB49 . TAIR Curator Summary Encodes a member of the basic leucine zipper transcription factor family, involved in ABA signalling during seed maturation and germination. The Arabidopsis abscisic acid (ABA)-insensitive abi5 mutants have pleiotropic defects in ABA response, including decreased sensitivity to ABA inhibition of germination and altered expression of some ABA-regulated genes.

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KEGG Orthology (KO) [BR:ath00001] 09130 Environmental Information Processing 09132 Signal transduction 04075 Plant hormone signal transduction AT1G03970 (GBF4)

International Journal of Molecular Sciences Review Linking Brassinosteroid and ABA Signaling in the Context of Stress Acclimation Victor P. Bulgakov 1,* and Tatiana V. Avramenko 1,2 1 Federal Scientiﬁc Center of the East Asia Terrestrial Biodiversity (Institute of Biology and Soil Science), Far Eastern Branch of the Russian Academy of Sciences, 159 Stoletija Str., Vladivostok 690022, Russia; KEGG Orthology (KO) [BR:ath00001] 09130 Environmental Information Processing 09132 Signal transduction 04075 Plant hormone signal transduction AT1G03970 (GBF4) The constitutive expression of ABI5 prevented the growth arrest of ppc2 under low CO2 conditions. The gene accession numbers in this study are available at TAIR (The Arabidopsis . (closed triangles), abi5-1 (open squares), and abi5-1 eel-1 (open cir- cles) were plated on medium supplemented with the indicated con- centrations of ABA, chilled for 4 days at 4 C in darkness, and At least seven isoforms (PsABI3-1 to PsABI3-7) of a putative, pea ABI3-like factor, originated by alternative splicing, have been identified after cDNA cloning. A similar variability had previously only been described for monocot genes. The full-length It is expressed in seeds and up-regulated in response to ABA. Loss of function mutants show decreased rate of germination in the presence of ABA. ABA dependent regulation is mediated by both ABI3 and ABI5. ABI5 promotes MFT expression, primarily in the radicle-hypocotyl transition zone and ABI3 suppresses it in the seed.